Phasor-based FLIM analysis of NAD(P)H and FAD autofluorescence for label-free bacterial classification.

ABSTRACT:

Significance

Bacterial contamination poses significant risks, particularly in medical settings, where timely identification is crucial for effective treatment. Traditional diagnostic methods often fail to provide rapid results, underlining the need for alternative approaches.

Aim

We investigate the potential of fluorescence lifetime imaging microscopy (FLIM) for fast label-free classification of bacterial species based on their growth states and intrinsic fluorescence characteristics.

Approach

Utilizing two-photon excitation microscopy, we examined the fluorescence lifetimes of the endogenous fluorophores NAD(P)H and FAD in Escherichia coli K12, Staphylococcus aureus, and Pseudomonas fluorescens. Cells were studied in different growth states-expon

SUBMITTER: Dyroy P 

PROVIDER: S-EPMC12811912 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature