Genetic Diversity of Neospora Caninum in Palestinian Cattle and Sheep: Insights From Nc-5 Gene Detection and MS10 Microsatellite Analysis.

ABSTRACT:

Introduction

Neosporosis is an infectious disease responsible for abortions in dairy cattle worldwide. This study aimed to detect Neospora caninum (N. caninum) in cattle and sheep in Palestine and to characterise the identified isolates genetically.

Methods

A total of 124 brain tissue specimens were obtained from slaughtered animals at Jericho abattoir, comprising 106 cattle (85%) and 18 sheep (15%). Polymerase chain reaction (PCR) targeting the Nc-5 gene and DNA Sanger sequencing were performed sequentially for the detection of N. caninum. The genetic variation and population structure were investigated using the MS10 microsatellite marker.

Results

Neospora caninum DNA was detected in 24.2% (30/124) of samples, with cattle showing a higher infection rate (25.5%) comp

SUBMITTER: Farajeen H 

PROVIDER: S-EPMC12812315 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature