Exposure of <i>in vitro</i> maturing pig oocytes to exogenous interleukin-6 is associated with transcriptomic differences in the resulting blastocysts, with enrichment of developmental signaling pathways.

ABSTRACT:

Introduction

Interleukin-6 (IL-6) has recently emerged as a promising supplement for in vitro maturation (IVM), enhancing pig blastocyst development and reducing apoptosis following in vitro fertilization (IVF). This study assessed whether IL-6 supplementation during IVM is associated with differences in gene expression in day-7 in vitro-produced blastocysts.

Methods

Oocytes from prepubertal gilts were matured in IVM medium with (IL-6 IVM group) or without (control group) 100 ng/mL IL-6 and subsequently subjected to IVF. The resulting presumptive zygotes were cultured in vitro for 7 days. Six pools of seven viable blastocysts per group were flash-frozen in liquid nitrogen and stored at -80 °C until microarray analysis. The embryos were analyzed us

SUBMITTER: Garcia-Canovas M 

PROVIDER: S-EPMC12812573 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature