Human donor islets coated with functionalized chitosan and alginate nanoscale layers can restore normoglycemia in diabetic mice.

ABSTRACT: Conformal coating is a promising encapsulation approach to reduce the immunogenicity of islet transplants in the treatment of type 1 diabetes (T1D). The primary objective of this work was to investigate a combination of non-immunogenic polyelectrolytes, tetrahydropyran triazole phenyl-alginate (TZ-AL) and quaternized phosphocholine-chitosan (PC-QCH), to create a nano-thin film onto the surface of human donor islets and assess its potential for clinical translation. To evaluate the coating materials, spheroids made from human-derived proliferative EndoC-βH1 and non-proliferative βH5 beta cell lines as pseudo-islet models was used as complementary models before applying the coating onto human islets. Coating formation and biocompatibility were first validated with spheroids and then applied

SUBMITTER: Yitayew MY 

PROVIDER: S-EPMC12813323 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature