Comparative Analysis of Extracellular Vesicle Isolation From Equine Serum and Plasma Using Two Isolation Methods With Structural and Proteomic Validation.

ABSTRACT: Extracellular vesicles (EVs) are promising biomarkers and mediators of intercellular communication, but their isolation from equine biofluids remains challenging. This study compared two isolation workflows-size-exclusion chromatography (SEC) and differential ultracentrifugation followed by SEC (UC + SEC)-to evaluate their efficiency, reproducibility, and the proteomic composition of EVs derived from equine serum and plasma. Blood from six healthy horses was processed to obtain platelet-free plasma and serum. EVs were isolated using SEC or UC + SEC and characterized by transmission electron microscopy, nanoparticle tracking analysis, and mass spectrometry-based proteomics with functional enrichment analysis. SEC provided higher reproducibility, greater protein yield, and a simpler workflow

SUBMITTER: Milczek-Haduch D 

PROVIDER: S-EPMC12813514 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature