Tregs Promote Astrocyte-Neuron Lactate Shuttle via Inhibiting STING Pathway to Improve Neurological Recovery After Ischemic Stroke.

ABSTRACT:

Background

Excessive immune response following ischemic stroke is closely associated with poor clinical prognosis. Although regulatory T cell (Treg) is recognized as pivotal immunomodulator, its potential mechanisms in post-stroke neurological recovery and immunotherapy remain unclear.

Methods

Neurological recovery and neuronal remodeling were investigated by behavior tests, HE staining, Nissl staining, and LFB staining. Monocarboxylate transporter (MCT) was detected to evaluate the role of astrocyte-neuron lactate shuttle (ANLS) in Tregs-mediated neuroprotection by immunofluorescence and western blot, lactate assay, ATP assay, and cell viability assay experiments. The expression of stimulator of interferon gene (STING) and phosphorylation of downstream factors were examined

SUBMITTER: Meng Y 

PROVIDER: S-EPMC12813866 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature