DNP-Enhanced Magic Angle Spinning Solid-State NMR Spectroscopy to Determine RNA-Ligand Interactions.

ABSTRACT: Understanding the molecular recognition underlying RNA-ligand complex formation is of key importance to explain the RNA regulatory function of riboswitches and to support the development of low-molecular-weight RNA binders as starting points for the development of RNA-targeting drugs. Here, we report magic angle spinning solid-state NMR spectroscopic studies enhanced by dynamic nuclear polarization (MAS-DNP) to determine the molecular recognition of a ligand-RNA riboswitch complex. We benchmarked different labeling strategies for four large RNAs (70-86 nt) of the aptamer domain of a 2'deoxyguanosine-sensing riboswitch from Mesoplasma florum. RNA samples were prepared either by chemoenzymatic approaches or by solid-phase chemical synthesis employing different labeling schemes of ribo

SUBMITTER: Sudakov A 

PROVIDER: S-EPMC12814176 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature