Fluorescence Quenching Properties and Bioimaging Applications of Readily Accessible Blue to Far-Red Fluorogenic Triazinium Salts.

ABSTRACT: Fluorogenic probes, which become fluorescent only upon specific activation, enable no-wash imaging with an excellent signal-to-noise ratio. Despite significant progress in the development of such probes, challenges remain in achieving efficient quenching and substantial fluorescence enhancement across the whole visible spectrum while maintaining good synthetic accessibility. In this work, we introduce a new class of bioorthogonally activatable fluorogenic probes based on triazinium salts (Trz⁺), which act as highly efficient fluorescence quenchers. These Trz⁺-fluorophore conjugates are easily synthesized from common precursors and commercially available fluorophores, covering a broad spectral range from blue to far-red wavelengths. Mechanistic studies provide molecula

SUBMITTER: Slachtova V 

PROVIDER: S-EPMC12814330 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature