Structural Analysis of Laminarin-Derived Oligosaccharides Produced by Transglycosylation of Exo-β-1,3-glucanase ScEXG1 from <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Exo-β-1,3-glucanase EXG1 (ScEXG1) from Saccharomyces cerevisiae hydrolyzes β-1,3-glucans and exhibits transglycosylation activity, enabling biocatalytic synthesis of prebiotic oligosaccharides. In this study, recombinant ScEXG1-6 × His was tested with laminaribiose as both the donor and acceptor. Under low-enzyme dosage, transglycosylation products were detected across a range of substrate concentrations (4.38-32.85 mM), with 21.9 mM laminaribiose producing 270.70 ± 10.32 nmol/mL of the main trisaccharide product-DP3 (DP3) after 3 h. Structural elucidation by HPLC-ESI-MS/MS identified DP3 as β-Glc-(1→6)-β-Glc-(1→3)-Glc based on glycosidic and cross-ring fragmentation patterns. Two additional tetrasaccharides were also characterized as β-Glc-(1→3)-β-Glc-(1→6)-β-Glc-(1→3)-Glc (DP4-1)

SUBMITTER: Kuo SY 

PROVIDER: S-EPMC12814337 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature