Intact Proteoform Analysis by Capillary Electrophoresis-Mass Spectrometry. Are We There Yet?

ABSTRACT: Mass spectrometry (MS)-based top-down proteomics (TDP) has emerged as a powerful tool for characterizing proteoforms to advance both fundamental and translational research. TDP requires high-efficiency liquid-phase separation, high-resolution MS, and tandem MS. Capillary zone electrophoresis (CZE)-MS has been proposed as a promising analytical technique for protein analysis decades ago because of its unique and valuable features, including high separation efficiency and high detection sensitivity. However, CZE-MS has not been widely adopted by the proteomics community, mainly due to concerns with its robustness and reproducibility. Here, we hypothesized that CZE-MS is sufficiently robust and reproducible for broad adoption due to the continued efforts of the community over the last three d

SUBMITTER: Gould N 

PROVIDER: S-EPMC12814393 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature