Artificial Multidomain Ribosomally Synthesized and Post-translationally Modified Peptide Enzymes for Farnesylated Peptide Library Generation.

ABSTRACT: Cyanobactin biosynthetic pathways are used in synthetic biology approaches to create large, peptide-based chemical libraries with drug-like features such as N-C macrocyclization and prenylation. It remains challenging to express enzymes from multiple RiPP pathways to rationally produce the desired products. Here, we designed a simple yet robust method aimed to produce and assess multiple enzymes, fusing biosynthetic genes together in a well-expressed, soluble construct that enables production of macrocyclic peptides and selectively appends C₅, C₁₀, or C₁₅ isoprenoids to tyrosine side chains. A library was developed and assayed, defining the sequence features necessary for prenylation and providing an overall >40% success rate of using a library with an esti

SUBMITTER: Lacerna N 

PROVIDER: S-EPMC12814516 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature