High-Throughput Detection of Cyanobacterial Form I Rubisco Assembly.

ABSTRACT: Rubisco catalyzes the CO₂ fixation step in the dark reactions of photosynthesis. Transgenic expression of better-performing Rubisco orthologs in plants or discovery of improved mutants of Rubisco via protein engineering could theoretically accelerate plant growth and improve crop yields. However, efforts to heterologously express or engineer Rubisco are frequently stymied by the chaperone-dependent folding and assembly of the Rubisco holoenzyme, a process that can be disrupted by changes to Rubisco's sequence. Elucidation of the effects that alterations to Rubisco's sequence impose upon its biogenesis is hampered by reliance upon low-throughput methods for verification of Rubisco assembly. Here, we report the engineering of a genetically encoded biosensor to sense the assembly o

SUBMITTER: Wysocki JW 

PROVIDER: S-EPMC12814518 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature