A Simple and Versatile Cell-Free Expression Method for Producing Secondary Metabolites.

ABSTRACT: Secondary metabolites are a major source of natural products with industrially relevant bioactivities. Lysate-based cell-free expression (CFE) is an emerging platform for accelerating the discovery and engineering of these natural products. While Escherichia coli cell extracts are widely used for CFE, Streptomyces extracts are likely to offer a more biochemically compatible environment for their expression. However, current Streptomyces-based CFE systems remain underdeveloped, with protocols that are either strain-specific or not readily scalable. To address these limitations and enable broader access to cell-free natural product biosynthesis, we present a generalizable and simple set of reaction conditions that support high-yield protein expression (180-230 μg/mL) in

SUBMITTER: Dinglasan JLN 

PROVIDER: S-EPMC12814521 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature