Characterizing Cell-Free Transcription and Translation Dynamics with Nucleic Acid-Based Assays.

ABSTRACT: Characterization of cell-free expression (CFE) systems must expand beyond single spectrophotometric measurements of a green fluorescent protein to provide meaningful metrics of system performance during a CFE reaction and enable the development of predictable and reproducible CFE technologies. To date, comprehensive characterization of these systems has posed a formidable measurement challenge, as it requires time-course measurements of reactions involving endogenous components in addition to transcription and translation of a target genetic circuit added exogenously to the CFE reaction. To provide more informative characterization that is still easy to conduct and complements current practices, we demonstrate a measurement framework for transcription and translation dynamics. We use diffe

SUBMITTER: Piorino F 

PROVIDER: S-EPMC12814534 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature