Tandem Mass Spectrometric Analysis and Cross-Species Comparison of Triacylglycerol Regioisomers in Mammalian Milk.

ABSTRACT: Milk fats have complex profiles of triacylglycerols (TG) with a significant gap in understanding the species-specific compositions of regioisomers. This study comprehensively analyzed TG regioisomers in milk of eight mammalian species using ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry and a calculation algorithm. Distinct TG regioisomer profiles were observed across species, shaped by the phylogenetic status and the structure of digestive tracts (ruminants, pseudo-ruminants, and non-ruminants). The ruminants (cow, goat, and sheep) exhibited highly similar milk TG regioisomeric profiles, while the pseudo-ruminant (camel) showed similar profiles to those of the non-ruminants (dog, horse, human, and pig). The non-ruminants showed a sn-2

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC12814545 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature