InSillyClo, a User-Friendly Web Application to Assist Large-Scale Golden Gate Cloning and MoClo Workflows.

ABSTRACT: Systems and synthetic biology developments often require the construction of many variants of a genetic circuit of interest, resulting in large-scale cloning campaigns. Golden Gate and Modular Cloning (MoClo), two powerful technologies enabling the scale-up of cloning workflows, play a central role for efficient circuit construction. These workflows include a number of dry-lab tasks, which are time-consuming and error-prone at scale. Currently, no software tool is available to handle these tasks in a dedicated, time-saving, and user-friendly manner. We present InSillyClo, an open-source web application to assist large-scale Golden Gate cloning and MoClo workflows. It supports an easy specification of genetic designs at any scale, followed by the automated generation of comprehensive workfl

SUBMITTER: Galez H 

PROVIDER: S-EPMC12814555 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature