Systematic Evaluation of Vector Sequence Elements in Isogenic CHO Cells for Improved Antibody Production.

ABSTRACT: Monoclonal antibodies (mAbs) constitute a leading class of biotherapeutics, and meeting global demand requires manufacturing platforms that deliver rapid and reliable expression. Nevertheless, efficient and predictable production remains challenging because multiple vector elements can substantially influence productivity and product quality. In this study, we examined how sequence design features influence expression in a targeted-integration CHO system using six antibodies (A-F). We evaluated codon usage in the constant light (CL) and constant heavy (CH) regions with and without LALA/YTE Fc mutations, the type of signal peptide, codon optimization of the variable regions, and substitution of the kappa light chain with lambda. We found that changing codons of CL and CH to suboptimal codon

SUBMITTER: Rahimi M 

PROVIDER: S-EPMC12814561 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature