Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection of <i>E. coli</i> in Drinking Water.

ABSTRACT: Modern genome editing methods permit the flexible modification of organisms at the genome level. However, bacteriophages, despite their small genomes, pose unique challenges due to the need to edit during their infection cycle, then select/screen for the modified genomes against a background of the wild type phage. Direct genome synthesis enabled by High-Complexity Golden Gate Assembly (HC-GGA) offers an alternative approach that permits rapid, accurate, and flexible genome modification. Here, we demonstrate HC-GGA's bacteriophage engineering potential, particularly in addressing the public health challenge of detecting hazardous pathogens and nonpathogenic bacteria as indicators of fecal contamination (indicator organisms) in water supplies. A bacteriophage-based biosensor was developed b

SUBMITTER: Parker DR 

PROVIDER: S-EPMC12814771 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature