Specific Detection of Prostate Cancer Cells in Urine by RNA In Situ Hybridization.

ABSTRACT:

Purpose

Noninvasive tests that can accurately detect prostate cancer are urgently needed for prostate cancer diagnosis, surveillance and prognosis. Exfoliated prostate cells captured in urine represent a promising resource for noninvasive detection of prostate cancer. We investigated performance of a novel cell-based urine test for detection of clinically significant prostate cancer.

Materials and methods

We previously developed a multiplex RNA in situ hybridization assay targeting NKX3-1, PRAC1 and PCA3 that enables identification and quantification of malignant and benign prostate cells released into urine. We investigated application of the assay for prostate cancer detection in a cohort of 98 patients suspected of harboring prostate cancer. Urine was collected following

SUBMITTER: Eskra JN 

PROVIDER: S-EPMC12814868 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature