Demonstration of the photo-controllability of the bistable opsin OPN5 using upconversion nanoparticles with multiple emission peaks upon near-infrared photoexcitation.

ABSTRACT: Upconversion nanoparticles (UCNPs) have emerged as promising tools for deep tissue optogenetics because they can convert near-infrared (NIR) light, which has high biopermeability, into ultraviolet (UV) or visible light. In optogenetics, microbially derived photoreceptor proteins such as channelrhodopsin (ChR) have been widely used. G protein-coupled receptor (GPCR)-type opsins have signal amplification capabilities and are emerging as promising candidates for high-sensitivity optogenetic tools. Since many GPCR-type opsins are so-called "bistable opsins" that can be switched between active and inactive states using light, it is not obvious whether multi-wavelength light from UCNPs can control bistable opsins. In this study, we constructed HEK293T cells expressing OPN5, a type of bistable op

SUBMITTER: Kotegawa F 

PROVIDER: S-EPMC12814955 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature