Chronic intermittent hypoxia exacerbates isoproterenol-induced cardiac hypertrophy and apoptosis.

ABSTRACT:

Background

Obstructive sleep apnea (OSA) is marked by chronic intermittent hypoxia (CIH) and iassociated with multiple cardiovascular complications. Isoproterenol (ISO) is commonly used to induce cardiac hypertrophy. However, the impact of CIH on ISO-induced cardiac hypertrophy and remodeling remains unclear.

Methods

Cardiac hypertrophy was induced in mice using ISO, with or without CIH. Echocardiography was performed to assess cardiac functions, while histological analyses were employed to evaluate the physiological modifications in the heart. Western blotting and real-time quantitative PCR were used to evaluate protein and mRNA gene expression levels, respectively. Additionally, immunofluorescence was employed to observe the morphological changes in H9C2 cells.

Results<

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12815717 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature