Crotonylation-related gene <i>GCDH</i> promotes osteoarthritis pathogenesis through flavin adenine dinucleotide signaling: mechanism exploration and experimental validation.

ABSTRACT:

Introduction

Osteoarthritis (OA) is a prevalent degenerative joint disorder characterized by progressive cartilage degradation and synovial inflammation. This study is designed to elucidate the role of crotonylation genes in OA progression.

Materials and methods

The crotonylation genes exposure and OA outcome data were obtained from the eQTLGen consortium and UK Biobank databases, respectively. Mendelian randomization analysis was employed to establish mechanistic links between crotonylation genes and OA, with subsequent validation conducted through cartilage RNA sequencing (RNA-seq) data. GCDH expression and its regulatory effects on key extracellular matrix (ECM) markers were assessed by Western blot (WB) and quantitative real-time polymerase chain reaction (qRT-PCR) in OA

SUBMITTER: Di J 

PROVIDER: S-EPMC12815788 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature