Dysregulated Tfh/B cells and their interactions in neuromyelitis optica spectrum disorder.

ABSTRACT:

Background

This study aimed to compare the proportions of circulating follicular helper T (Tfh) and B cell subsets, as well as serum levels of cytokines and chemokines, between patients with neuromyelitis optica spectrum disorder (NMOSD) who are anti-aquaporin-4 antibody (AQP4-ab)-positive and healthy controls, and to investigate the interaction mechanisms between Tfh and B cells.

Methods

AQP4-ab-positive NMOSD patients were enrolled during acute attacks and remission phases, along with age- and sex-matched healthy controls. Flow cytometry was used to assess circulating Tfh and B cell subsets. Purified CD19⁺ B cells were cultured alone or co-cultured with CD4⁺CXCR5⁺ Tfh cells for 6 days, with various interventions applied to evaluate alterati

SUBMITTER: Wang L 

PROVIDER: S-EPMC12815817 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature