Photodynamic therapy with methylene blue effectively kills antibiotic resistant bacteria from pediatric patients with perforated appendicitis.

ABSTRACT: Perforated appendicitis (PA) is the most common cause of intra-abdominal abscess in children and is associated with higher costs, extended hospitalizations, and worse postoperative outcomes compared to uncomplicated appendicitis. Photodynamic therapy (PDT) utilizes a photosensitizer and light to generate cytotoxic reactive species that are efficacious against bacteria. We sought to determine whether PDT could effectively eliminate bacteria isolated from the peritoneal cavities of pediatric patients with perforated appendicitis. After IRB approval, bacterial subcultures from 30 pediatric surgical patients with PA were developed as planktonic monocultures. Samples were derived from standard clinical collection of peritoneal fluid in patients undergoing surgery for PA. Cultures were incubated

SUBMITTER: Wirth KT 

PROVIDER: S-EPMC12815967 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature