Environmental DNA reveals the distinct genetic diversity and evolutionary pathways of the Chinese Minnow Rhynchocypris oxycephalus in Korean freshwater systems.

ABSTRACT: Establishing an environmental DNA (eDNA) reference library at regional and local scales is essential not only for accurate biodiversity assessment but also for comprehensive long-term monitoring. To date, genetic diversity studies of the Chinese minnow (Rhynchocypris oxycephalus) have largely been restricted to China, leaving substantial knowledge gaps across its broader distribution, including South Korea. Hence, the present study identified suitable regions for guiding eDNA surveys and non-invasive sampling, based on documented occurrences retrieved from the IUCN Geospatial Conservation Assessment Tool (GeoCAT). The newly designed primer pairs successfully amplified long mitochondrial fragments (~ 1 kb) of the cytochrome b (Cytb) and 16S ribosomal RNA (16S rRNA) genes. The generated sequ

SUBMITTER: Bang G 

PROVIDER: S-EPMC12815971 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature