Metagenomic insights into effect of pulse cell wall integrity on gut microbiota, CAZyme gene responses and starch/protein metabolism during in vitro fecal fermentation.

ABSTRACT: The physical structure of pulse cotyledon cells modulates gut microbiota by controlling starch and protein availability for colonic fermentation, yet the mechanisms governing the interplay between saccharolytic and proteolytic fermentation remain unclear. Here, enzymatically treated white kidney bean cotyledon cells with weakened cell walls (CWs) underwent in vitro fecal fermentations and shotgun sequencing. Impaired CWs enhanced fermentation, increased acetate and propionate production, and reduced branched-chain fatty acids (BCFAs) and ammonia. Damaged CWs upregulated CAZymes encoding genes GH4, GH15, GH126, CBM20, and CBM26, which are associated with amylase, α-glucosidases, and amyloglucosidase activities involved in starch degradation. Furthermore, amino acid pathway enrichment reveal

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12816062 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature