Visualizing intracellular milieu and electric field-induced cell responses by fluorescence lifetime imaging microscopy.

ABSTRACT: Fluorescence-based optical techniques have significantly advanced our comprehension of living systems, providing invaluable insights into cellular intricacies and facilitating fundamental research and practical diagnostic applications. To explore intracellular environments and the communication among intracellular organelles, we utilized fluorescence lifetime imaging microscopy with exogenous fluorophores of Oregon Green 488 BAPTA-1 AM and Fluovolt and endogenous fluorophores of NAD(P)H and FAD in breast carcinoma MCF7 and MDA-MB-231 and normal MCF10A cells. Fluorescence lifetime images have uncovered a characteristic triangular interrelationship among intracellular calcium ion concentration (Ca²⁺), mitochondrial function, and membrane electric potential in cancerous and normal

SUBMITTER: Awasthi K 

PROVIDER: S-EPMC12816114 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature