BMP4 dose dictates lineage specification bias in human periodontal ligament stem cells.

ABSTRACT:

Introduction

Periodontal ligament stem cells (PDLSCs) represent a promising therapeutic cell source for regenerating periodontal tissues impaired by disease. While exogenous BMP4 has been used to induce human PDLSC differentiation into distinct periodontal cell lineages, its dose-dependent effects on lineage specification remain poorly understood.

Methods

Here, we systematically investigated how BMP4 dosage modulates human PDLSC differentiation toward osteogenic and tenogenic lineages using single-cell RNA sequencing and in vitro functional assays.

Results

Single-cell transcriptomic analysis revealed that endogenous BMP4 expression inversely correlated with RUNX2 but positively correlated with SCX within PDLSC subpopulations. Pseudotemporal

SUBMITTER: Li Y 

PROVIDER: S-EPMC12816240 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature