Immunogenicity of subunit vaccine of E2 protein against atypical porcine pestivirus in pigs.

ABSTRACT:

Introduction

An atypical porcine pestivirus (APPV) causes congenital tremor (CT) type A-II in piglets and has caused considerable financial losses to the pig industry worldwide. This study aimed to develop an effective vaccine candidate against APPV.

Methods

We constructed a prokaryotic expression system for the APPV E2 protein in Escherichia coli. The immunogenicity of the APPV E2 subunit vaccine, formulated with three different adjuvants (ISA 201VG, IMS 1313VG, and Gel 02), was evaluated in piglets. Assessment was based on E2-specific antibody levels measured by an in-house enzyme-linked immunosorbent assay (ELISA) and protection against an APPV challenge. Successful expression of the E2 protein was confirmed by western blot analysis.

Results

The E2 protein was succ

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12816379 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature