Genomic characterization, antimicrobial resistance, and virulence profiling of <i>Escherichia coli</i> isolated from diarrheic calves in Gansu, China.

ABSTRACT:

Introduction

This study provides a comprehensive genomic investigation of Escherichia coli isolated from diarrheic calves in Gansu Province, China, a region with significant livestock production.

Methods

We employed whole-genome sequencing on 15 isolates from 15 different farms to characterize their molecular subtypes, plasmid repertoires, virulence gene profiles, and antibiotic resistance mechanisms.

Results

Our analysis revealed high genetic diversity with 10 sequence types and 9 serotypes, including a novel serogroup. Phenotypic testing demonstrated widespread multidrug resistance, yet canonical resistance genes were absent in many resistant strains. Phylogenetic analysis elucidated the roles of both clonal dissemination and horizontal gene transfer.

Disc

SUBMITTER: Meng Q 

PROVIDER: S-EPMC12816387 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature