Integrated bioinformatics and tissue-based validation reveal the oncogenic role of hsa_circ_0043256 and hsa_circ_0004789 in gastric cancer.

ABSTRACT: Circular RNAs (circRNAs) play a key role in gastric cancer (GC) pathogenesis. This study hsa_circ_0043256 and hsa_circ_0004789, and their interactions with miR-28-5p/Cyclin B1 (CCNB1) and miR-5683/CCNB1, via bioinformatic and experimental methods. We retrieved expression data for circRNAs, miRNAs, and mRNAs in GC from Gene Expression Omnibus and The Cancer Genome Atlas. Using online databases and R tools, we identified downstream miRNAs and target mRNAs to build a competing endogenous RNA (ceRNA) network. After identification of hub genes and performing functional enrichment, we defined two regulatory axes: hsa_circ_0043256/miR-28-5p/CCNB1 and hsa_circ_0004789/miR-5683/CCNB1. We studied 32 paired tumor and adjacent tissues to assess all genes and CCNB1 protein expression, along with correl

SUBMITTER: Aslani S 

PROVIDER: S-EPMC12816855 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature