Depletion of individual dietary amino acids induce distinct metabolic and chromatin states.

ABSTRACT: Reducing dietary levels of protein or specific essential amino acids (EAAs) promote favorable metabolic reprogramming, including improved glucose tolerance, increased insulin sensitivity, and reduced fat mass. However, the extent to which shared or EAA-specific mechanisms facilitate diet-associated phenotypes remains unclear. Here, we compared the physiological and molecular responses to dietary levels of methionine, leucine, and isoleucine by feeding C57BL/6J mice diets in which each of these specific AAs is depleted. Dietary depletion of Met, Leu, or Ile (Met-D, Leu-D, or Ile-D) elicited distinct, AA-specific physiological and hepatic molecular (transcriptome, metabolome, histone proteome) responses that were not phenocopied by mTORC1 inhibition via rapamycin treatment. Ile-D yielded the

SUBMITTER: Haws SA 

PROVIDER: S-EPMC12816912 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature