Gamma-FIT-PNAs as sensitive RNA probes.

ABSTRACT: A variety of oligonucleotide-based probes have been developed for specific and selective sensing of RNA and DNA. Among these, FIT-PNAs (forced intercalation-peptide nucleic acids) and FIT probes (DNA- and RNA-based sensors) have been studied for a variety of RNA biomarkers in cell culture and tissues, and in vivo. FIT-PNAs and FIT probes are RNA/DNA sensors that exhibit fluorescence upon sequence-specific RNA/DNA hybridization. Several synthetic approaches have been successfully applied to increase the brightness and selectivity of these molecules, including the introduction of cyclopentane (cp) modified PNA monomers (cpPNA) as well as locked nucleic acids (LNAs-for FIT probes). In this report, we have explored the biophysical properties of FIT-PNAs that are modified with gamma-l-se

SUBMITTER: Gupta MK 

PROVIDER: S-EPMC12817130 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature