Porcine Packed Red Blood Cells Demonstrate a Distinct Red Blood Cell Storage Lesion.

ABSTRACT:

Introduction

The red blood cell (RBC) storage lesion has been well described in mouse and human blood but not in swine. Understanding the porcine RBC storage lesion is necessary prior to evaluating transfusion of stored packed red blood cells (pRBCs) in polytrauma models. We hypothesized that porcine pRBCs would undergo a similar storage lesion severity after 42 d.

Methods

Whole blood was collected from female Yorkshire pigs and pRBCs were isolated in additive storage solution 3. Female human whole blood was obtained from our local blood bank and pRBCs prepared. Human and porcine pRBCs were stored for 42 d and sampled weekly and evaluated for markers of the RBC storage lesion including biochemical measurements, eryptotic RBCs, band-3 expression, erythrocyte-derived microvesi

SUBMITTER: Chae RC 

PROVIDER: S-EPMC12817170 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature