Cocaine-Induced Changes in the BRD4 Interactome Identifies Casein Kinase 1 Epsilon as a Therapeutic Target.

ABSTRACT: Recent research has revealed that BRD4, a bromodomain and extra terminal domain (BET) epigenetic "reader" protein, plays an essential role in regulating behavioral and molecular responses to cocaine. To date, the roles of BRD4 and other BET proteins in substance use disorder (SUD) models have been mainly studied using small molecule inhibitors that block the bromodomain interactions with acetylated histones. In other disease models, non-bromodomain BRD4-protein interactions have also been shown to play an important role in BRD4's molecular functions, but these interactions have yet to be studied in SUD models. Here, using BRD4 co-immunoprecipitation coupled to tandem mass spectrometry, we identified several putative BRD4-interacting proteins in the nucleus accumbens (NAc) that are altered

SUBMITTER: Seyednejad A 

PROVIDER: S-EPMC12817219 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature