Dacarbazine as a Positive Control in Melanoma Cell Lines (A375, SK-MEL-103, 1205Lu) and a Human Ex Vivo Skin Model.

ABSTRACT: One important step for evaluating and selecting a drug is toxicity studies, which are responsible for eliminating molecules that are considered promising for treating a certain disease based on their effectiveness in clinical studies, but are unsafe to go to the pharmaceutical market. We proposed an evaluation of dacarbazine as a positive control in toxicity effects in the context of macro- and micro effects represented by tissue and cell responses. A resazurin assay is used to evaluate cytotoxicity in cells (melanoma cells A375, Sk-Mel-103, and 1205Lu; immortalized skin cells HaCat and 3T3), and hematoxylin/eosin staining and TUNEL staining are used in skin explants. There is no toxicity demonstrated in the immortalized cells at the studied concentrations, whereas in the melanoma cells, A

SUBMITTER: Leite MN 

PROVIDER: S-EPMC12817238 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature