Validation of a model of rheumatoid arthritis using mice reconstituted with patient peripheral blood mononuclear cells.

ABSTRACT: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by inflammation and joint destruction. Replicating human manifestations of RA in animal models remains challenging, however, owing to heterogeneity of the disease. In this study, a humanized mouse model for RA was developed and validated using NOD-scid IL2Rγnull (NSG) mice engrafted with peripheral blood mononuclear cells (PBMCs) from patients with RA (NSG-RA). RA symptoms were induced using lipopolysaccharide and a cocktail of antibodies against type II collagen. Pathological manifestations were assessed through clinical scoring of hind paw swelling, histological analysis, and evaluation of RA-specific markers in plasma and joints using Luminex, RT-PCR and RNA sequencing. NSG-RA mice exhibited increased levels of RA-s

SUBMITTER: Schuster-Winkelmann P 

PROVIDER: S-EPMC12817335 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature