NaMeco - Nanopore full-length 16S rRNA gene reads clustering and annotation.

ABSTRACT:

Background

Nanopore sequencing is currently one of the leading third-generation sequencing technologies on the market and is gaining popularity among researchers. Due to its long-read capabilities, full-length 16S rRNA gene metabarcoding using Oxford Nanopore Technologies (ONT) offers great potential for metataxonomic studies. However, the relatively high error rate poses a significant challenge for bioinformatic processing, often limiting taxonomy resolution to the genus level despite the longer read length.

Results

This study presents NaMeco, a novel tool specifically developed to efficiently process long 16S rRNA gene reads sequenced using Oxford Nanopore Technologies, requiring minimal user input. Our tool performs read quality control, primer-specific extraction of sequ

SUBMITTER: Yergaliyev T 

PROVIDER: S-EPMC12817509 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature