N-acetylglucosamine facilitates coordinated flow-like movement of myoblasts, forming a foundation for efficient myogenesis.

ABSTRACT:

Background

Skeletal muscle comprises 30-40% of a mammal's body mass, maintaining its integrity through efficient muscle fiber regeneration, which involves myoblast differentiation into myotubes. Previously, we reported that N-acetylglucosamine (GlcNAc) promotes myogenesis in C2C12 cells, although the underlying processes remained unclear. GlcNAc's activated form, UDP-GlcNAc, is critical for the biosynthesis of highly branched (N-acetyllactosamine-rich) N-linked oligosaccharides, which are recognized by galectin-3 (Gal-3), a protein that facilitates dynamic cell-cell and cell-matrix interactions and modulating the motility dynamics of membrane-associated proteins.

Methods

In this study, we used primary myoblasts from both wild-type and Gal-3 null (Gal-3KO) mice, observing myo

SUBMITTER: Satoh MS 

PROVIDER: S-EPMC12817516 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature