Ecological success in freshwater lakes: insights from novel cultivated lineages of the abundant Nanopelagicales order.

ABSTRACT:

Background

The order Nanopelagicales is the most abundant bacterioplankton lineage in freshwater lakes and exhibits typical streamlined genomic characteristics such as small cell volumes (<0.1 μm³), reduced genome sizes (<1.5 Mbp), and low GC content. These characteristics reflect adaptations to a free-living life strategy in oligotrophic environments. While many Nanopelagicales metagenome-assembled genomes and single-amplified genomes are available in public databases, strain-level microdiversity within this lineage remains poorly understood. This is mainly attributed to the incomplete nature of these genomes and the difficulty in isolating and maintaining pure cultures, with only 20 genome-sequenced cultures available to date.

Results

Here, we report the isolati

SUBMITTER: Chiriac MC 

PROVIDER: S-EPMC12817590 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature