Targeting the LINC01272-FUS signal axis inhibits the migration and invasion of testicular germ cell tumors.

ABSTRACT:

Objective

Testicular germ cell tumors (TGCTs), the most prevalent malignancy in males aged 15-40 years, exhibit suboptimal prognosis despite therapeutic advances. This study aimed to identify novel diagnostic biomarkers and elucidate their molecular mechanisms in TGCTs pathogenesis.

Methods

Through integrated bioinformatics analysis of public TCGA(The Cancer Genome Atlas)datasets and clinical specimen validation (n = 14), we screened LINC01272 as a candidate biomarker. RNA immunoprecipitation (RIP) assays were employed to verify the interaction between LINC01272 and fused in sarcoma (FUS). Functional experiments including siRNA-mediated gene silencing, transwell migration/invasion assays, and CCK-8 proliferation tests were performed in TGCTs cell lines (Tcam-2 and NCCIT).

SUBMITTER: Zhao X 

PROVIDER: S-EPMC12817599 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature