Eriocitrin inhibits sodium iodate-induced cuproptosis and barrier function impairment in retinal pigment epithelium via SIRT7/YAP/ATP7A pathway.

ABSTRACT:

Background

Dry age-related macular degeneration (AMD) is characterized by retinal pigment epithelium (RPE) barrier dysfunction, and currently lacks effective treatment options. Eriocitrin is a natural flavonoid with antioxidant and anti-inflammatory properties, and its potential role in inhibiting cuproptosis and improving RPE barrier function remains unclear.

Methods

ARPE-19 cells treated with sodium iodate (NaIO₃) were used to establish an in vitro AMD model. The effects of eriocitrin at various concentrations (0-100 µM) and treatment durations on cell viability were assessed using the CCK-8 assay. ELISA and ROS fluorescence were used to assess inflammation and oxidative stress levels. Western blotting and qPCR analysis were employed to evaluate the expression of copper-de

SUBMITTER: Wang X 

PROVIDER: S-EPMC12817798 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature