AND logic-gated CRISPR/Cas9 and hybridization chain reaction system for precise ctDNA detection.

ABSTRACT: Circulating tumor DNA (ctDNA) is a critical biomarker for liquid biopsies, enabling the non-invasive acquisition of cancer-related information from blood samples. Precise detection of ctDNA, particularly the identification of single-nucleotide variations (SNVs), is crucial for early cancer diagnosis, therapeutic monitoring, and prognostic evaluation. However, current ctDNA detection methods often encounter challenges such as complex procedures, difficult data analysis, and false-positive signals during pre-amplification. In this study, we introduce a novel detection method based on AND logic-gated integration of interspaced short palindromic repeats and associated proteins (CRISPR/Cas9) system with hybridization chain reaction (HCR) isothermal amplification. This strategy enhances the spec

SUBMITTER: Ji T 

PROVIDER: S-EPMC12817871 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature