Orthogonal chemical genomics approaches reveal genomic targets for increasing anaerobic chemical tolerance in <i>Zymomonas mobilis</i>.

ABSTRACT: Genetically engineered microbes have the potential to increase efficiency in the bioeconomy by overcoming growth-limiting production stress. Screens of gene perturbation libraries against production stressors can identify high-value engineering targets, but follow-up experiments needed to guard against false positives are slow and resource-intensive. In principle, the use of orthogonal gene perturbation approaches could increase recovery of true positives over false positives because the strengths of one technique compensate for the weaknesses of the other, but, in practice, two parallel screens are rarely performed at the genome scale. Here, we screen genome-scale CRISPRi (CRISPR interference) knockdown and transposon insertion libraries of the bioenergy-relevant Alphaproteobacterium,

SUBMITTER: Eckmann JB 

PROVIDER: S-EPMC12817903 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature