Exposure single-cell metabolomics mass spectrometry reveals HFPO-DA toxicity mechanisms.

ABSTRACT: Hexafluoropropylene oxide-dimer acid (HFPO-DA) is widely used in food packaging bags, surfactants, lubricants, etc. As a new substitute for the persistent organic pollutant perfluorooctanoic acid (PFOA), it has been proven to have potential health and environmental risks similar to other perfluorinated compounds. However, studies based on the average levels of many cells or tissues cannot accurately reflect the toxic mechanism of HFPO-DA. Here, we construct a high-throughput exposure single-cell metabolomics mass spectrometry analysis platform based on intact living-cell electrolaunching ionization MS analysis, optimize the detection conditions of the platform, and use it to investigate the effects of a wide range of HFPO-DA concentrations on the metabolism of mouse embryonic stem c

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12817909 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature