Single-cell omics uncover gene dynamics shaping embryonic and extra embryonic lineages in pig blastocysts.

ABSTRACT: Late blastocyst development before implantation is a unique feature of ungulates, during which the epiblast proliferates and maintains pluripotency while extra-embryonic tissues expand dramatically, elongating to several tens of centimeters. The mechanisms coordinating these processes are not well understood. We performed single-cell omics profiling of porcine blastocysts from the hatched stage (E7) through early (E9) and late ovoid stages (E11). From 15,370 cells, we identified distinct embryonic and extra-embryonic populations with characteristic chromatin accessibility profiles. We reconstructed gene regulatory networks using enhancer-based eRegulons and validated them through motif occupancy analysis. Extra-embryonic tissues showed strong shifts in gene regulatory module activity at th

SUBMITTER: Dufour A 

PROVIDER: S-EPMC12818244 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature