Bioactive molecules unearthed by terabase-scale long-read sequencing of a soil metagenome.

ABSTRACT: Metagenomics provides access to the genetic diversity of uncultured bacteria through analysis of DNA extracted from whole microbial communities. Long-read sequencing is advancing metagenomic discovery by generating larger DNA assemblies than previously possible. However, harnessing the potential of long-read sequencing to access the vast diversity within soil microbiomes is hampered by the challenge of isolating high-quality DNA. Here we introduce a method that can liberate large, high-quality metagenomic DNA fragments from soil bacteria and pair them with optimized nanopore long-read sequencing to generate megabase-sized assemblies. Using this method, we uncover hundreds of complete circular metagenomic genomes from a single soil sample. Through a combination of bioinformatic prediction a

SUBMITTER: Burian J 

PROVIDER: S-EPMC12818350 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature