Understanding Biases in Liquid-Liquid Phase Separation: Investigating Amino Acid Enrichments in Phase-Separating Proteins toward Peptide Design.

ABSTRACT: Liquid-liquid phase separation (LLPS) facilitates the formation of membraneless organelles, enhancing biochemical processes. The stickers-and-spacers model explains LLPS but is mainly validated in prion-like RNA-binding proteins. To broaden our understanding, we investigated peptide motifs associated with LLPS across diverse protein contexts using a computational approach on the droplet-promoting regions (DPRs) of 178 phase-separating proteins. The study identified 129 enriched peptide motifs (3-6 residues), characterized by Gly-rich sequences interspersed with aromatic, charged, and polar residues, as well as homopeptide repeats (e.g., GGDR, SRGG, QQQQ). Analysis of motif presence and frequency revealed a widespread distribution across DPRs and significant repetitive patterns. Motif trios

SUBMITTER: Calvario J 

PROVIDER: S-EPMC12818744 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature