Graphitic Carbon Nitride: A Reusable and Stable Support for Glucose Oxidase.

ABSTRACT: This study demonstrates the successful immobilization of the enzyme glucose oxidase (GOx) onto 2D thermally exfoliated graphitic carbon nitride (GCN-T) via physical adsorption, producing a stable, reusable, and highly active bioconjugate. Binding was confirmed by scanning transmission electron microscopy (STEM) coupled with energy-dispersive spectroscopy (EDS), thermogravimetric analyses (TGAs), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). At the optimal conditions, GCN-T/GOx showed an immobilization yield of 88% and an activity of 0.54 U mg^-1. Immobilized GOx retained 81% of its initial activity over 10 consecutive cycles and 50% after 10 storage/reuse cycles. While the free enzyme lost 27% of activity at 70 °C, the immobilized enz

SUBMITTER: Barros RAM 

PROVIDER: S-EPMC12818753 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature