<i>In Silico</i> Structural Analysis of the Grapevine Serine Protease VviSBT4.19 Involved in Defense against <i>P. viticola</i>.

ABSTRACT: Grapevine pathogens, such as Plasmopara viticola, a downy mildew disease-causing agent, pose a significant threat to grape and wine production worldwide. Molecular details are needed on how tolerant grapevine species recognize and mount a successful defense response against this pathogen. We have identified the important role of grapevine subtilase VviSBT4.19; however, without a reliable structural model, we have a limited understanding of protease activity modulation by P. viticola. Here, we built a consensus computational model of this protein and performed constant-pH MD simulations to assess its overall structural stability. We identified Ser419 as the key residue in autocatalytic processing and demonstrated that its S419N mutation produces a stable, active protease with

SUBMITTER: Rodrigues FEP 

PROVIDER: S-EPMC12818760 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature